Welcome to my Personal Webpage

Shrawan Kumar Upadhyay

Shrawan Kumar Upadhyay

    • Sex
    • :
    • Male

    • D.O.B
    • :
    • 1987-11-16
    • Phone
    • :
    • +9779813044275
    • Address
    • :
    • Malpara, 4-Kapilvastu
      32800, Nepal
    • E-mail
    • :
    • shrawankumar_44@yahoo.com

Summary


Self-motivated and goal-oriented Biochemistry Graduate having 8+ years of formal education in Biochemistry and expertise in protein engineering work. Passionate about learning new research techniques and ability of working hard beyond office hours. Demonstrated experience in Protein and DNA isolation and analysis, site-directed mutagenesis and analysis of Hypocholesterolemic activities.


Work Experience


Master's Thesis from December 2016 to November 2017
Biochemistry Lab - IC-UPLB, Los Banos, Laguna, Philippines

  • Designed mutation sites and primers for site-directed mutagenesis
  • Carried out site-directed mutagenesis on 8Sa globulin to introduce Hypocholesterolemic peptide, LPYPR
  • Successfully expressed, isolated and purified recombinant protein
  • Analyzed Hypocholesterolemic activity of the recombinant 8Sa globulin for cholesterol binding and bile-acid binding activities
  • Successfully accomplished introduction of the Hypocholesterolemic peptide, LPYPR, into the 8Sa globulin


  • Research Student from January 2017 to November 2017
    Biochemistry Lab - IC-UPLB and Nanobiotech Lab - UPLB-BIOTECH, Los Banos, Laguna, Philippines

  • Successfully isolated and purified reductase from plant growth promoting bacteria
  • Analyzed reductase using PAGE and its role in formation of gold nanoparticles
  • Designed different enzymatic assays to identify the identity of the reductase
  • Estimated production of the indole-3-acetic acid by plant growth promoting bacteria
  • Compared different intermediates of the indole-3-acetic acid pathway using thin layer chromatography
  • Conducted substrate feeding assay for the determination of root of Indole-3-acetic acid pathway

  • Molecular Biology Lab Student from August 2015 to December 2015
    MBB Lab - IBS-UPLB, Los Banos, Laguna, Philippines

  • Isolated plasmid and Genomic DNA from bacteria found in pitcher fluid of Nepenthes
  • Amplified the gene encoding 16S rRNA using PCR
  • Analyzed bacterial community by conducting density gradient gel electrophoresis of the nested-PCR product of the gene encoding 16S rRNA

  • Biochemistry Lab Student from August 2014 to December 2014
    Biochemistry Lab - IC-UPLB, Los Banos, Laguna, Philippines

  • Isolated and purified Major Storage Protein of Mungbean using gel filtration chromatography
  • Isolated and characterized xyloglucans and lipids from Tamarind seeds


  • Education


    Master of Science in Biochemistry - UPLB, Los Banos, Laguna, Philippines from August 2014 to January 2018
  • Master's Thesis: Introduction of the Hypocholesterolemic Peptide, LPYPR, to the Major Storage Protein of Mungbean [Vigna radiata (L.) Wilczek] Through Site-Directed Mutagenesis

  • Bachelor of Science in Biochemistry - Pokhara University, Kaski, Nepal from August 2008 to July 2013
  • Bachelor's Thesis: Isolation, Purification and Characterization of Protease from Different Parts of Choreospondias axillaries (Lapsi)

  • Relevant coursework: Analytical Biochemistry, Bioinformatics, Metabolism I & II, Biomolecules I & II, Biostatistics, Biotechnology, Carbohydrate Chemistry, Protein Chemistry, Cell Biology, Microbiology, Molecular Biology, Plant Biochemistry, Physical Chemistry I & II, Organic Chemistry I & II, Inorganic Chemistry I & II

  • Research Interest


  • Cancer research
  • Genetic engineering
  • Membrane trafficking and signal transduction
  • Molecular aspect of neurodegenerative diseases
  • Plant biochemistry and molecular biology

  • Research Experience


  • Isolation, Purification and Characterization of the Reductase for the Biosynthesis of Gold Nanoparticles by Plant Growth Promoting Bacteria, Nanobiotech Lab - UPLB-BIOTECH, Philippines, Supervisor: Dr. Lilia M. Fernando
  • Isolation, Purification and Characterization of the Key Enzyme Involved in the Synthesis of Indole-3-Acetic Acid by Plant Growth Promoting Bacteria, Nanobiotech Lab - UPLB-BIOTECH, Philippines, Supervisor: Dr. Lilia M. Fernando
  • Microbial Community Analysis from the Pitcher Fluid of Nepenthes using Nested-PCR-DGGE Analysis of Genes Encoding 16S rRNA, MBB Lab - IBS-UPLB, Philippines, Supervisor: Dr. Nacita B. Lantican
  • Isolation, Purification and Characterization of Xyloglucans and Lipids from Tamarind (Tamarindus indica) Seeds, Biochemistry Research Lab - IC-UPLB, Philippines, Supervisor: Dr. Mariam C. Recuenco
  • Isolation, Purification and Characterization of Major Storage Proteins from Cowpea (Vigna unguiculata) Seeds, Biochemistry Research Lab - IC-UPLB, Philippines, Supervisor: Dr. Mary Ann O. Torio

  • Publications


  • Upadhyay, S. K., Magar, R. T., & Thapa, C. J. (2013). Biochemical Characterization of Protease Isolated from Different Parts of Choerospondias axillaris (Lapsi). Biochem Anal Biochem, 2(135), 2161-1009


  • Workshops and Training Activities


  • Seminar-Workshop on Basics of Molecular Biology held on 25th of November 2016 at Interactive Learning Center, NCAS Building, UPLB, Philippines
  • Short term training on Molecular Genetics at Kathmandu Center for Genomics and Research Laboratory, Lalitpur, Nepal, from January 26 to February 05, 2014
  • Short term 2 day workshop on "Refresher Course on Spectroscopic Techniques for Graduate Students" organized by Nepal Chemical Society, Kathmandu, Nepal, June 13 - 14, 2009

  • Skills


  • Statistical analysis: STAR, GraphPad Prism
  • Scientific Software: PyMol, SWISS-MODELING, Image LabTM, BioEdit, ImageJ
  • Computer skills: MS Office (Word, Excel, Powerpoint)
  • Laboratory equipments: HPLC, Agarose and polyacrylamide gel electrophoresis, Standard and high-speed centrifugation, UV-Visible and Nanodrop Spectrophotometer, Gel Doc. System, Incubators and Biosafety Cabinet, Column Chromatography using Gel, Ion exchange and Hydrophobic resins, qPCR
  • Biochemical Assays: Hypocholesterolemic activities, Protein and DNA analysis and quantification, Enzyme assays
  • Bioinformatic analysis: BLAST, Primer designing

  • Honors and Awards


  • July 12, 2018: Best Scientific Poster Award (40th Annual Scientific meeting, National Academy of Science and Technology, Philippines)

  • References


    Available upon request